HPLC + MS
Independently tested for identity and purity
NP-5AMQ-02
Aug 6, 2026
99.67%
View Certificate of Analysis
CAS# 42464-96-0
Formula C₁₀H₁₁N₂·I
MW 286.11 Da
Target NNMT (IC₅₀ 1.2 μM)
Form Powder
Molecular Weight
Molecular Formula
Compound Class
5-Amino-1MQ (5-amino-1-methylquinolin-1-ium iodide, CAS 42464-96-0) is a synthetic quinolinium small molecule and selective substrate-site inhibitor of nicotinamide N-methyltransferase (NNMT), with a reported IC₅₀ of 1.2 μM. It is studied in vitro for its effects on the NAD⁺ salvage pathway, methionine cycle flux, and metabolic enzyme activity in cell culture models. Supplied by NexPept as powder for in vitro laboratory research use.
Molecular Profile
CAS#, formula, molecular weight, analytical data
CAS#, formula, molecular weight, analytical data
| Property | Value |
|---|---|
| CAS Number | 42464-96-0 |
| Full Name | 5-amino-1-methylquinolin-1-ium iodide |
| Molecular Formula | C₁₀H₁₁N₂·I |
| Molecular Weight | 286.11 g/mol |
| Sequence | N/A — small molecule |
| Chain Length | N/A — small molecule |
| Compound Class | Synthetic quinolinium salt / NNMT inhibitor |
| Physical Form | Powder |
| Analytical Method | High-Performance Liquid Chromatography (HPLC) + Mass Spectrometry (MS) |
| Appearance | White to off-white powder |
In Vitro Research Context
NNMT inhibition / NAD⁺ salvage pathway — laboratory use only
NNMT inhibition / NAD⁺ salvage pathway — laboratory use only
5-Amino-1MQ selectively occupies the substrate binding site of nicotinamide N-methyltransferase (IC₅₀ = 1.2 μM), blocking the enzyme’s N-methylation of nicotinamide. Substrate-site targeting distinguishes it from cofactor-competitive inhibitors.
NNMT competes with NAMPT for nicotinamide substrate. In vitro inhibition by 5-Amino-1MQ is studied for its effects on nicotinamide flux and intracellular NAD⁺ pool dynamics in metabolic cell culture models.
NNMT consumes S-adenosylmethionine (SAM) as a methyl donor during nicotinamide methylation. Inhibition preserves SAM availability, affecting transmethylation reactions throughout the methionine cycle in cell models.
In vitro studies have investigated 5-Amino-1MQ in adipogenesis models, including reduced lipogenesis in 3T3-L1 cells (EC₅₀ = 30 μM) and decreased 1-methylnicotinamide levels (EC₅₀ = 2.3 μM), as well as in skeletal muscle cell regeneration and metabolic enzyme activity research.
Research context: 5-Amino-1MQ is used as a tool compound in preclinical cell culture research investigating NNMT enzyme activity and its downstream effects on NAD⁺ metabolism and cellular methylation flux.
Available Formats
Powder — in vitro research use
Powder — in vitro research use
| Format | Net Quantity | Physical Form |
|---|---|---|
| 5-Amino-1MQ | Per vial | Powder |
Sourced from a verified batch. Quantity for use should be determined based on your specific in vitro research protocol by qualified research personnel.
Laboratory Storage
Controlled storage conditions for powder material
Controlled storage conditions for powder material
Store sealed powder material under controlled laboratory conditions in accordance with validated institutional SOPs and applicable technical documentation. Protect from moisture and light. Handling conditions should be established by qualified research personnel in accordance with the specific experimental protocol.
Note: No preparation, reconstitution, or administration instructions are provided. This compound is supplied for in vitro laboratory research use only. All handling must be conducted by qualified personnel following institutional safety protocols.
Research Literature
Scientific references & related resources
Scientific references & related resources
PubMed — 5-Amino-1MQ / NNMT Inhibition — peer-reviewed scientific literature
Research Peptides in Canada — sourcing and research context for Canadian laboratories
What Are Research Peptides? — compound classification and in vitro research context
This product is supplied exclusively for in vitro laboratory and non-clinical research use by qualified research personnel. It is not authorized by Health Canada for human or veterinary use. No therapeutic, clinical, or health claims are made.
This material must not be administered to humans or animals. No dosage, preparation, reconstitution, or administration instructions are provided or implied. All handling and use must be conducted by qualified personnel in accordance with applicable institutional policies and legal requirements.
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