HPLC + MS
Independently tested for identity and purity
NP-NAD500-062426-01
Jul 16, 2026
99.39%
View Certificate of Analysis
CAS# 53-84-9
Full Name Nicotinamide Adenine Dinucleotide
Class Coenzyme / Redox Cofactor
Form Lyophilized Powder
Molecular Weight
Cofactor Role
Molecular Formula
NAD⁺ (Nicotinamide Adenine Dinucleotide, CAS# 53-84-9) is a naturally occurring dinucleotide coenzyme found in all living cells, functioning as an essential redox cofactor in cellular energy metabolism. It participates as an electron carrier in key metabolic pathways and serves as a substrate for enzymes including sirtuins (SIRTs) and poly(ADP-ribose) polymerases (PARPs). Supplied by NexPept as a lyophilized powder for in vitro laboratory research use.
Molecular Profile
CAS#, formula, molecular weight, analytical data
CAS#, formula, molecular weight, analytical data
| Property | Value |
|---|---|
| CAS Number | 53-84-9 |
| Full Name | Nicotinamide Adenine Dinucleotide (oxidized form) |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular Weight | 663.43 g/mol |
| Compound Class | Dinucleotide coenzyme — redox cofactor |
| Enzyme Substrates | Sirtuins (SIRT1–7), PARPs, CD38, NMNAT |
| Physical Form | Lyophilized powder |
| Analytical Method | High-Performance Liquid Chromatography (HPLC) + Mass Spectrometry (MS) |
| Appearance | White to off-white lyophilized powder |
In Vitro Research Context
Metabolic pathways & enzyme substrates — laboratory use only
Metabolic pathways & enzyme substrates — laboratory use only
Functions as an electron carrier in glycolysis, the TCA cycle, and oxidative phosphorylation research models.
The NAD⁺/NADH ratio is studied as a key indicator of cellular redox state in in vitro assay systems.
NAD⁺ is a required substrate for SIRT1–7 deacetylase activity, studied in epigenetic and metabolic regulation assays.
Serves as the ADP-ribose donor substrate for poly(ADP-ribose) polymerases in DNA repair and stress response research.
NAD⁺ is a widely used biochemical tool in laboratory research due to its central role in cellular metabolism and enzymatic regulation. Research applications include metabolic flux analysis, enzyme activity assays, mitochondrial function studies, and investigation of NAD⁺-dependent signalling pathways in controlled in vitro settings.
Research context: NAD⁺ is a naturally occurring coenzyme studied extensively in preclinical and in vitro research settings involving cellular metabolism, redox biology, and enzyme substrate characterisation.
Available Formats
500mg — lyophilized powder
500mg — lyophilized powder
| Format | Net Quantity | Physical Form |
|---|---|---|
| NAD⁺ 500mg | 500 mg / vial | Lyophilized powder |
Sourced from a verified batch. Quantity for use should be determined based on your specific in vitro research protocol by qualified research personnel.
Laboratory Storage
Controlled storage conditions for lyophilized material
Controlled storage conditions for lyophilized material
Store sealed lyophilized material under controlled laboratory conditions in accordance with validated institutional SOPs and applicable technical documentation. NAD⁺ is hygroscopic and sensitive to moisture and light — protect from both at all times. Handling conditions should be established by qualified research personnel in accordance with the specific experimental protocol.
Note: No preparation, reconstitution, or administration instructions are provided. This compound is supplied for in vitro laboratory research use only. All handling must be conducted by qualified personnel following institutional safety protocols.
Research Literature
Scientific references & related resources
Scientific references & related resources
NAD⁺ Canada — NexPept Overview — compound overview and in vitro research context
PubMed — NAD⁺ / Nicotinamide Adenine Dinucleotide — peer-reviewed scientific literature
Research Peptides in Canada — sourcing and research context for Canadian laboratories
What Are Research Peptides? — compound classification and in vitro research context
This product is supplied exclusively for in vitro laboratory and non-clinical research use by qualified research personnel. It is not authorized by Health Canada for human or veterinary use. No therapeutic, clinical, or health claims are made.
This material must not be administered to humans or animals. No dosage, preparation, reconstitution, or administration instructions are provided or implied. All handling and use must be conducted by qualified personnel in accordance with applicable institutional policies and legal requirements.
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